Plasmid

Part:BBa_K4354011:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-29)


plasmid pUC19


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 423
    Illegal PstI site found at 435
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal PstI site found at 435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 417
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 423
    Illegal PstI site found at 435
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 423
    Illegal PstI site found at 435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1766
    Illegal SapI site found at 683


Design Notes

We need to cut off the lacZ promoter and replace it with a strong constitutive promoter P43.


Source

Our lab has stocked it for a period of time.

References